Figure 6. Comparison of shearing in candidate regions.

A. Gel image for samples prepared from wild-type and sir1Δ, sir2Δ strains and the blots hybridized for ACT1, HMRa1, and the input-Seq under-covered sub-telomeric region. B. The data in the blots were quantified by analyzing the signal intensity for each fragment and plotted as counts (y-axis) against fragment size (x-axis). The plots compare the size distribution of Sir+ and Sir− cells for each probe. C. Gel image for samples prepared from wild-type strains only and the blots hybridized for ACT1 (average input-Seq coverage), TRA1 (under-covered), and the over-covered promoter. D. Plots, as in B, comparing size distributions of ACT1 as a control to the TRA1 and promoter distributions.