Table 1. Statistical characteristics of V4 and V6 amplicon sequence analysis from the four fecal samples, at two different similarity levels.

Datasets	ALL-V4		A-V4-0.5		B-V4-0.5		A-V6-1.0		B-V6-1.0		C-V4-0.5		D-V4-0.5		C-V4-0.1		D-V4-0.1
Untrimmed reads	860,597		213,294		235,489		402,823		405,130		206,365		205,449		47,100		44,907
Trimmed reads	740,704		185,003		194,316		267,104		260,042		176,106		185,279		42,315		40,741
Similarity	97%	98%	97%	98%	97%	98%	97%	98%	97%	98%	97%	98%	97%	98%	97%	98%	97%	98%
Phylotype richness	5,249	11,623	1,686	3,621	1,472	3,074	12,049	14,533	12,545	14,738	1,629	3,701	1,778	3,467	574	1,103	678	1,186
Chao1 richness estimation	7,847	17,408	2,584	5,310	1,977	4,217	18,950	25,993	19,045	25,976	2,461	5,286	2,675	5,300	931	1,655	1,000	1,805
Chao1-LCI95	7,563	16,942	2,415	5,076	1,868	4,038	18,466	25,281	20,027	25,293	2,302	5,062	2,513	5,045	828	1,528	913	1,671
Chao1-UCI95	8,165	17,915	2,793	5,581	2,115	4,429	19,470	26,752	19,470	26,704	2,657	5,548	2,872	5,597	1,077	1,818	1,120	1,975
Shannon diversity	6.400	8.105	4.913	5.678	4.864	5.678	7.004	7.108	7.038	7.123	4.365	5.413	3.743	4.496	3.626	4.389	3.344	4.178
Species evenness	0.747	0.866	0.661	0.693	0.667	0.707	0.745	0.742	0.746	0.742	0.590	0.659	0.500	0.552	0.571	0.626	0.513	0.590
Good's coverage	99.72%	99.45%	99.65%	99.31%	99.76%	99.51%	98.00%	97.28%	97.83%	97.12%	99.69%	99.41%	99.62%	99.31%	99.42%	99.01%	99.33%	98.83%