A: Representative photographs from three independent experiments of TNF-α protein expression in the descending colon in the various experimental groups at selected time points: day 7, day 28, day 56 continued injection subgroup, day 56 discontinued injection subgroup, day 70 continued injection subgroup and day 70 discontinued injection subgroup. Band intensity was adjusted for the corresponding GAPDH, and values were expressed as arbitrary density units (ADU); B: TNF-α protein expression in the descending colon in the various experimental groups both in the continued and discontinued injection subgroups, in response to treatment at all time points. Note the significant difference in expression between the IA + B and the other groups (P < 0.005).