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. Author manuscript; available in PMC: 2010 Aug 18.
Published in final edited form as: Biochemistry. 2009 Aug 18;48(32):7565–7567. doi: 10.1021/bi901006b

Figure 1.

Figure 1

Incision by UvrABC on substrates 5a (A), 5b (B), 5c (C), and 5d (D). Lanes 1–3 are identical in A–D. Lane 1: G+A sequencing, lane 2: G sequencing, and lane 3: UvrABC reaction. The decomposition products formed prior to incision are indicated by the blue arrow. The inset (A) highlights the minor products of 5a, which are a result of incision on the unlabeled strand. (E) Histogram of incision sites. Relative intensities are shown by red arrows. (F) Cartoon depiction of anomalously slow migrating incision products 6a and 6b formed from cleavage of nicked strand in 5b and 5c, respectively. Radiolabeled position is indicated (*)