Figure 2.
Summary of PER.C6® cell line generation. The expression construct is introduced into serum-free and suspension adapted PER.C6® cells by electroporation. Following a short recovery period, cells are seeded into 96-well plates by limiting dilution, whereupon stable transfectants are selected by survival in the presence of the antibiotic Geneticin®. Following productivity screens in the multi-well plates, candidate cell lines are expanded and cultured in shake flasks. Batch and fed-batch assays were performed on the candidate cell lines to determine Qpmax and volumetric productivity.