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. 2009 Mar-Apr;1(2):128–141. doi: 10.4161/mabs.1.2.7631

Figure 4.

Figure 4

Characterization of stability-engineered BHA10 scFvs. (A) Binding activity of wild-type BHA10 scFv, stability-engineered BHA10 scFvs, and BHA10 FAb to LTβR was measured following thermal challenge. Binding profiles are normalized to 100% maximum binding. The temperature at which 50% binding activity is retained (T50, °C) is indicated. Samples were assayed in duplicate. (B) Temperature-dependent binding of the hydrophobic dye ANS to BHA10 scFvs. PBS, (○); wild-type BHA10 scFv (containing (Gly4Ser)3 linker), (●); BHA10-GS4 scFv (▴); and BHA10-SS/GS4 scFv (▪).