Skip to main content
. 2009 Mar-Apr;1(2):128–141. doi: 10.4161/mabs.1.2.7631

Figure 5.

Figure 5

Design and expression of stability-engineered BsAbs. (A) Western blot analysis of BsAbs transiently expressed in CHO cells. Supernatant samples in the left panel are analyzed under non-reducing conditions and under reducing conditions in the right panel. Lane 1: MW marker, 2: C-BsAb, 3: C-BsAb-GS4, 4: C-BsAb-SS, 5: C-BsAb-SS/GS4, 6: N-BsAb, 7: N-BsAb-GS4, 8: N-BsAb-SS, 9: N-BsAb-SS/GS4. Arrow indicates presence of ∼55–60 kDa unidentified immunoreactive species. (B) Normalized SEC profiles of stability-engineered C-BsAbs following Protein A chromatography. Sharp peaks eluting between 13–15 min represent monomeric BsAb. Broad peaks eluting between ∼10.75–14 min represent aggregated protein. Blue dash-dot = C-BsAb, red dash = C-BsAb-SS, and green line = C-BsAb-SS/GS4.