Table 1.

Binding constants of affinity maturated anti-hNKG2D mAbs in comparison with the human precursor mAb E1

Human antibody	kd (1/s)	ka (1/Ms)	KD = kd/ka (M)
E1 mAb	6.1 ± 0.9 × 10−4	4.4 ± 0.6 × 104	1.7 ± 0.5 × 10−9
C3 mAb	1.0 ± 0.0 × 10−6	3.1 ± 0.1 × 105	3.3 ± 0.3 × 10−11
C12 mAb	2.1 ± 0.7 × 10−6	2.9 ± 0.1 × 105	7.3 ± 2.1 × 10−11
H5 mAb	1.5 ± 0.0 × 10−6	2.7 ± 0.5 × 105	5.8 ± 1.0 × 10−11
B1 mAb	9.9 ± 2.9 × 10−6	4.3 ± 0.4 × 105	2.3 ± 0.4 × 10−11
E4 mAb	9.3 ± 4.5 × 10−6	3.5 ± 0.3 × 105	2.7 ± 1.4 × 10−11

Rates of association (ka) and dissociation (kd) of the different human anti-hNKG2D Abs were measured by surface plasmon resonance (SPR) and the equilibrium dissociation constant K_D [M] was calculated from kd/ka. The values shown are means ± standard deviation of three independent Biacore experiments.