Table 2.
Characteristics of mAb E4
| Characteristic | Unit | E4 mAb |
| Isotype | - | Human IgG1λ |
| Identity of human VL to human germline | - | 89% identity to human VL_13-7(A)1c |
| Identity of humanized VH to human germline | - | 88% identity to human VH 4-59 |
| Specifity | - | Human and macaque NKG2D |
| Binding affinity | Equilibrium dissociation constant (KD) | 2.7 ± 1.4 × 10−11 M (BiaCore) |
| Association rate constant, ka | 3.5 ± 0.3 × 105 M−1s−1 | |
| Dissociation rate constant, kd | 9.3 ± 4.5 × 10−6 s−1 | |
| Inhibition of NK2D-dependent cytotoxicity | Half maximal inhibitory concentration (IC50) | 0.22 ± 0.08 nM |
| Induction of NK2D-dependent cytotoxicity | Half maximal effective concentration (EC50) | 19.40 ± 11.80 pM |
| Serum stability at 37°C | - | at least 7 days |
| Heat stability | - | Up to 70°C |
| Inhibition of cytokine release | - | yes |
| Induction of cytokine release | - | yes |
| Internalization by NKL cells | - | yes |