Figure 1.

Immunofluorescence staining with 8H8 mAb of dengue infected cells with Den-2 A15 strain. C6/36-HT cells were infected with Den-2 A15. At 6 (a), 8 (b), 12 (c), 48 (d) and 72 (e) h p.i, the infected cells were reacted 8H8 mAb followed with an FITC-conjugated goat anti-mouse antibody and then examined under a fluorescence microscope. Cytoplasmatic and nuclear staining of infected cells are indicated as C and N, respectively. C6/36-HT cells infected with Den-2A15 reacted with anti-Den-2 polyclonal antibody at 72 h p.i was used as a positive control (f) and non-infected C6/36-HT cells were used as negative control (g).