Table 3.
Max distance m |
SNm (%) |
SPm (%) |
TPm | FNm | FPm |
---|---|---|---|---|---|
0 | 99.98 | 99.15 | 1 493 582 | 316 | 12 781 |
3 | 99.99 | 99.29 | 1 500 662 | 186 | 5831 |
5 | ~100 | 99.72 | 1 502 428 | 67 | 4184 |
10 | ~100 | 99.79 | 1 503 481 | 54 | 3144 |
Using a threshold of 30, Figaro is able to remove virtually all vector sequence and only overtrims a small proportion of reads by more than 3 bp. Note false positives and false negatives are computed only if they occur in the high-quality region of a read.