Figure 4.

Zic2 induces a greater increase in ipsilateral RGC projections compared with EphB1. A, Low-power (top) and high-power (bottom) images of E18.5 retina electroporated with GFP plus Zic2 at E13.5. Note that the majority of GFP⁺ cells stain positive for Zic2. B, Representative examples of E18.5 retina and OTs from embryos electroporated with GFP, GFP plus EphB1, and GFP plus Zic2 at E13.5. C, Whereas electroporating EphB1 at E13.5 did not induce a greater increase in GFP⁺ ipsilateral (Ipsi) projections compared with E14.5 electroporations, ectopic Zic2 expression is significantly more efficient in converting contralateral (Contra) RGC projections to an ipsilateral fate. n = 5 for GFP control electroporations and n ≥ 10 for EphB1 and Zic2 electroporations, from three or more different electroporation experiments. Data represent mean ± SEM. Scale bars, 100 μm. ANOVA: F_(2,25) = 71.28, p < 0.0001; modified t tests: **p < 0.005, ***p < .0001. NF, Neurofilament.