TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Feature(s) | Source or reference |
|---|---|---|
| Strains | ||
| Shewanella oneidensis | ||
| MR-1 | Wild-type strain | ATCC |
| PSRA1 | In-frame deletion mutant | This study |
| PSRA+ | “Knock-in” complementation mutant | This study |
| Escherichia coli | ||
| EC100D pir-116 | F−mcrA Δ(mrr-hsdRMS-mcrBC) | Epicentre |
| φ80dlacZΔM15 ΔlacX74 recA1 endA1 | ||
| araD139 Δ(ara leu)7697galUgalK λ− | ||
| β2155 λ pir | thrB1004 pro thi strA hsdS lacZΔM15 | 12 |
| (F9 lacZΔM15 lacIqtraD36 proA1 proB1) | ||
| ΔdapA::erm pir::RP4 Kmr | ||
| Plasmids | ||
| pKO2.0 | 4.5-kb γR6K, mob RP4 sacB GmrlacZ | This study |
| pKNOCK-Gm | 1.6-kb γR6K, mob RP4 Gmr | 2 |
| pBBR1MCS | CmrlacZ | 31 |
| pJQ200 | ColE1 sacB Gmr | 48 |
| pKOPSRA | pKO2.0 containing in-frame deletion of psrA | This study |
| pKOPSRA+ | pKO2.0 containing wild-type psrA | This study |