FIG. 3.

Western blot analysis of MtrC standard, TM, and whole cells (WC). MtrC content was visualized by using affinity-purified polyclonal antibodies to MtrC. MtrC protein standard amounts ranged from 1.8 × 10⁻¹¹ to 1.8 × 10⁻¹² moles and were subjected to 10% SDS-PAGE along with TM fractions (0.5 μg per lane) and whole-cell samples (7.5 μl of cell suspension [2.25 × 10⁷ total cells]).