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. 2009 Jun 19;75(16):5218–5226. doi: 10.1128/AEM.00544-09

FIG. 6.

FIG. 6.

Kinetic simulation of OmcA-mediated reduction of goethite in the presence of riboflavin. The reaction was monitored by measuring the oxidation of ferroOmcA as absorbance (Abs) at 550 nm. Data points are from experiments with 0.47 μM ferroOmcA, 1 μM flavin, and various concentrations of goethite, i.e., 21 (•), 34 (▪), and 113 (▴) mM. Lines represent simulated data for the reaction mechanism shown in Fig. 8A with rate constants of 5.9 × 105 M−1 s−1 for OmcA and flavin and 0.22 M−1 s−1 for flavin and goethite, reactant concentrations of 0.476 μM OmcA and 1 μM riboflavin, and the three goethite amounts indicated above.