TABLE 1.
Kinetic constants for MtrC with soluble and insoluble iron formsa
| Expt | Second-order rate constant (M−1 s−1), mean ± SD
|
|||
|---|---|---|---|---|
| EDTA-Fe3+ | NTA-Fe3+ | Citrate-Fe3+ | Goethite | |
| Purified enzyme | 9.2 × 105 ± 3.9 × 104 | 2.4 × 105 ± 3.7 × 104 | 3.9 × 104 ± 3.3 × 103 | 0.0099 ± 0.0007 (0.0033 ± 0.0003)b |
| TM | 1.9 × 104 ± 2.5 × 103 | 1.3 × 104 ± 2.7 × 103 | 1.2 × 103 ± 3.0 × 102 | 14.7 ± 2.7 (4.9 ± 0.9) |
| Whole cells | 9.0 × 103 ± 1.0 × 103 | 1.1 × 104 ± 1.7 × 103 | 6.7 × 103 ± 4.4 × 102 | 1.37 ± 0.17 (0.45 ± 0.05) |
a
See Materials and Methods for experimental details.
b
The values in parentheses are in milliliters per square meter per second.