TABLE 2.
Kinetic constants for OmcA with soluble and insoluble iron formsa
| Expt | Second-order rate constant (M−1 s−1), mean ± SD
|
|||
|---|---|---|---|---|
| EDTA-Fe3+ | NTA-Fe3+ | Citrate-Fe3+ | Goethite | |
| Purified enzyme | 3.0 × 105 ± 7.4 × 103 | 1.7 × 105 ± 2.9 × 104 | 3.7 × 104 ± 3.2 × 103 | 0.019 ± 0.003 (0.0066 ± 0.001)b |
| TM | 1.5 × 104 ± 2.1 × 103 | 1.4 × 104 ± 4.0 × 103 | 1.2 × 103 ± 3.0 × 102 | 24.2 ± 5.5 (8.1 ± 1.8) |
| Whole cells | 9.2 × 103 ± 1.1 × 103 | 1.1 × 104 ± 1.5 × 103 | 7.0 × 103 ± 4.6 × 102 | 1.15 ± 0.2 (0.38 ± 0.07) |
a
See Materials and Methods for experimental details.
b
The values in parentheses are in milliliters per square meter per second.