TABLE 1.

Expression levels and solubilities of human proteins with or without a PrS₂ tag

NESG identification no.a	No. of residues	pET		pCold His6 tag		pCold-PST
		Expressionb	Solubilityc	Expressionb	Solubilityc	Expressionb	Solubilityc
HR79	168	+	+	0	NAd	++	+
HR2898	165	++++	+	++	+	++++	++++
HR2921	134	+++	0	+	0	+	+
HR2929	182	++++	+	++++	+++	+++++	+++++
HR2930	175	+	+	0	NA	++	+
HR3073	184	++	0	++	0	+++	+++
HR3111	119	0	NA	0	NA	+++	+++

^aHR79, human Ras-like transcription suppressor; HR2898, human growth arrest and DNA damage-inducible protein GADD45-alpha, involved in apoptosis, DNA repair, nuclear localization, and regulation of cyclin-dependent kinase activity, has limited sequence similarity to ribosomal protein L30; HR2921, human DNA-binding inhibitor ID-2, required for G₁ progression; HR2929, human HSP20 heat shock protein beta 2, protein chaperone, enzyme activator; HR2930, Bcl2-related protein A1 (human Bcl-2-related protein, hematopoiesis-specific early response protein, Glasgow rearranged sequence protein); HR3073, human Bcl2-modifying protein, involved in apoptosis, myosin-binding protein; HR3111, human DNA-binding inhibitor ID-3, transcriptional corepressor (http://nmr.cabm.rutgers.edu:9090/PLIMS/targetViewerQuickSearchContent.jsf; 1).

^bExpression levels (where 100% indicates total protein expression by E. coli) are semiquantitatively indicated from 0 (not detected) and + (>5%) to +++++ (∼50%).

^cProtein solubility is also indicated from 0 to +++++ as described in footnote b.

^dNA, not applicable.