TABLE 3.
Coordinates of phage CB19 ORFs and representative ORFs and genes in the other 936-type phagesa
| Strand | ORF | Start | Stop | Size (aa) | MM (kDa) | pI | SD sequence AGAAAGGAGGTb | Representative ORF or gene (% aa identity) in indicated phage
|
Putative function | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SL4 | CB13 | CB14/GR7 | CB20 | P008 | bIL170 | sk1 | jj50 | 712 | bIBB29 | |||||||||
| + | 1 | 266 | 790 | 174 | 19.9 | 5.1 | AGAAAGGATAAtATG | ORF1 (98) | ORF1 (98) | ORF1 (100) | ORF1 (98) | ORF1 (96) | L1 (97) | ORF1 (97) | ORF1 (97) | ORF1 (97) | ORF1 (95) | Terminase small subunit |
| + | 2 | 787 | 1017 | 76 | 9.0 | 4.0 | GCACCAGAGGGatttgaATG | ORF2 (96) | ORF2 (97) | ORF2 (100) | ORF2 (97) | |||||||
| + | 3 | 1029 | 2651 | 540 | 63.0 | 6.0 | AGAAAGGTAATgaATG | ORF3 (98) | ORF3 (97) | ORF3 (100) | ORF3 (97) | ORF2 (97) | L2 (95) | ORF2 (97) | ORF2 (98) | ORF2 (97) | ORF2 (97) | Terminase large subunit |
| + | 4 | 2641 | 2925 | 94 | 11.2 | 9.1 | AGAAATGGCGGtgtcagATG | ORF4 (98) | ORF4 (100) | ORF4 (100) | ORF4 (100) | ORF3 (95) | L3 (97) | ORF3 (96) | ORF3 (96) | ORF3 (96) | ORF3 (98) | HNH endonuclease |
| + | 5 | 2938 | 4074 | 378 | 43.3 | 5.0 | AGAAAGGGGAAaaaTTG | ORF5 (96) | ORF5 (97) | ORF5 (95) | ORF5 (100) | ORF4 (94) | L4 (94) | ORF4 (92) | ORF4 (92) | ORF4 (92) | ORF4 (96) | Portal protein |
| + | 6 | 4055 | 4591 | 178 | 19.9 | 4.6 | AGAAAGGACGTaacaagcacagATG | ORF6 (98) | ORF6 (96) | ORF6 (99) | ORF6 (100) | ORF5 (98) | L5 (98) | ORF5 (98) | ORF5 (97) | ORF5 (97) | ORF5 (97) | Prohead protease |
| + | 7 | 4584 | 5765 | 393 | 43.7 | 5.5 | ATTGAGGATATtaaaaagaaatATG | ORF7 (93) | ORF7 (98) | ORF7 (96) | ORF7 (100) | ORF6 (95) | L6 (95) | ORF6 (95) | ORF6 (95) | ORF6 (96) | ORF6 (94) | Minor structural protein |
| + | 8 | 5786 | 6049 | 87 | 10.0 | 6.2 | AAAACGGAGGAagtaaATG | ORF8 (98) | ORF8 (96) | ORF8 (96) | ORF8 (100) | ORF8 (98) | L8 (98) | ORF7 (98) | ORF7 (96) | ORF7 (97) | ORF7 (96) | |
| + | 9 | 6049 | 6363 | 104 | 11.8 | 9.0 | ATTATGGAGGTatttaATG | ORF9 (95) | ORF9 (94) | ORF9 (92) | ORF9 (100) | ORF9 (94) | L9 (90) | ORF8 (92) | ORF8 (96) | ORF8 (93) | ORF8 (95) | |
| + | 10 | 6353 | 6691 | 112 | 12.9 | 4.2 | AGAGGGGGTCGtaagtaATG | ORF11 (94) | ORF10 (96) | ORF10 (92) | ORF10 (100) | ORF10 (91) | L10 (93) | ORF9 (96) | ORF9 (91) | ORF9 (91) | ORF9 (93) | |
| + | 11 | 6682 | 7047 | 121 | 13.7 | 10.2 | GTGCAGGTGGTcaaccATG | ORF12 (97) | ORF11 (95) | ORF11 (93) | ORF11 (100) | ORF11 (96) | L11 (94) | ORF10 (98) | ORF10 (95) | ORF10 (95) | ORF10 (95) | |
| + | 12 | 7104 | 9074 | 656 | 71.3 | 5.2 | ACAATAATGGTattttttaATG | ORF12 (33) | ORF12 (40) | ORF12 (100) | ORF12 (63) | L12 (85) | ORF12 (53), ORF16 (18) | NPS | ||||
| + | 13 | 9098 | 10003 | 301 | 32.6 | 4.9 | AAAAAGGAAAAtaaaaaATG | ORF13 (94) | ORF13 (91) | ORF13 (96) | ORF13 (100) | ORF13 (92) | L13 (93) | ORF11 (93) | ORF11 (92) | ORF11 (85) | ORF11 (85) | Major capsid protein |
| + | 14 | 10041 | 10316 | 91 | 10.6 | 4.9 | TAAAGGGATATaaaacaaaATG | ORF14 (98) | ORF14 (100) | ORF14 (97) | ORF14 (100) | ORF14 (100) | L14 (100) | ORF12 (98) | ORF12 (100) | ORF13 (100) | ORF13 (97) | |
| + | 15 | 10336 | 10848 | 170 | 19.9 | 4.8 | TGAGAGGGCTGtgaATG | ORF15 (97) | ORF15 (96) | ORF15 (97) | ORF15 (100) | ORF15 (97) | L15 (98) | ORF13 (95) | ORF13 (98) | ORF14 (95) | ORF14 (97) | |
| + | 16 | 10848 | 13838 | 996 | 105.6 | 9.1 | AGAAAGGGTATgtaATG | ORF16 (97) | ORF16 (86) | ORF16 (85) | ORF16 (100) | ORF16 (75) | L16 (76) | ORF14 (82) | ORF14 (81) | ORF15 (74) | ORF15 (77) | TMP |
| + | 17 | 13838 | 14734 | 298 | 34.4 | 5.5 | ACTAGGGAGGGcttaATG | ORF17 (92) | ORF17 (94) | ORF17 (100) | ORF17 (100) | ORF17 (92) | L17 (91) | ORF15 (92) | ORF15 (92) | ORF16 (86) | ORF16 (95) | |
| + | 18 | 14734 | 15861 | 375 | 42.8 | 5.1 | AGAAAGGCGGActtcgtttaATG | ORF18 (94) | ORF18 (94) | ORF18 (95) | ORF18 (100) | ORF18 (92) | L18 (91) | ORF16 (91) | ORF16 (91) | ORF17 (91) | ORF17 (92) | |
| + | 19 | 15851 | 16144 | 97 | 11.4 | 9.3 | AGAAAGTGGAGacaaaccaaATG | ORF19 (97) | ORF19 (97) | ORF19 (100) | ORF19 (100) | ORF19 (96) | L19 (96) | ORF17 (96) | ORF17 (94) | ORF18 (96) | ORF18 (96) | |
| + | 20 | 16134 | 16943 | 269 | 29.1 | 5.7 | TCAAGAAAGGTtaaaaATG | ORF20 (88) | ORF20 (87) | ORF20 (100) | ORF20 (100) | ORF20 (45) | L20 (46) | ORF18 (75) | ORF18 (76) | ORF19 (59) | ORF19 (32) | RBP |
| + | 21 | 16965 | 17318 | 117 | 13.5 | 6.3 | AGAAAGCAAAAtaaaATG | ORF21 (88) | ORF21 (96) | ORF21 (96) | ORF21 (100) | ORF21 (90) | L21 (87) | ORF19 (92) | ORF19 (90) | ORF20 (91) | ORF20 (89) | Holin |
| + | 22 | 17315 | 18019 | 234 | 26.0 | 5.7 | CAAACGGAGGAtaaaaaagaATG | ORF22 (98) | ORF22 (100) | ORF22 (100) | ORF22 (100) | ORF22 (76) | L22 (76) | ORF20 (67) | ORF20 (68) | ORF21 (68) | ORF21 (76) | Endolysin |
| − | 23 | 18613 | 18464 | 49 | 6.0 | 8.5 | CAACTAGAAGTatagcgTTG | ORF23 (89) | ORF23 (59) | ORF23 (100) | ORF23 (100) | ORF23 (61) | E36 (85) | p21 (91) | ORF23 (83) | ORF22 (87) | ||
| − | 24 | 18940 | 18674 | 88 | 10.2 | 4.6 | AAATTAAAGGTatgaatagATG | ORF25 (96) | ORF24 (93) | ORF24 (100) | ORF24 (100) | ORF25 (93) | E33 (93) | ORF21 (95) | ORF21 (95) | ORF24 (85) | ORF25 (90) | |
| − | 25 | 19460 | 19113 | 115 | 13.5 | 9.1 | ATAATTGAGGTtatagcataATG | ORF26 (85) | ORF25 (79) | ORF25 (100) | ORF25 (100) | ORF26 (66) | E31 (65) | ORF23 (90) | ORF22 (90) | ORF26 (73) | ORF27 (81) | |
| − | 26 | 19627 | 19460 | 55 | 6.9 | 5.8 | AGACAGGAGTAatcggataATG | ORF27 (90) | ORF26 (90) | ORF26 (100) | ORF26 (100) | ORF27 (90) | E30 (90) | ORF24 (35) | ORF23 (35) | ORF27 (35) | ORF28 (42) | |
| − | 27 | 19803 | 19627 | 58 | 6.8 | 10.3 | AGAAAGCTAGTgaataatATG | ORF28 (96) | ORF27 (91) | ORF27 (100) | ORF27 (100) | ORF28 (88) | E29 (90) | ORF29 (90) | ||||
| − | 28 | 19996 | 19805 | 63 | 7.1 | 10.2 | AGAAAGTTTTGgtgaaaaaataaATG | ORF29 (93) | ORF28 (88) | ORF28 (100) | ORF28 (100) | |||||||
| − | 29 | 20295 | 20038 | 85 | 9.8 | 5.0 | GAAAAGGAGGTtaaataGTG | ORF30 (91) | ORF29 (96) | ORF29 (100) | ORF29 (100) | ORF29 (68) | E28 (65) | ORF25 (88) | ORF24 (86) | ORF28 (75) | ORF30 (69) | |
| − | 30 | 20676 | 20368 | 102 | 12.1 | 4.6 | GGCTTGGAGGTaacatctaATG | ORF31 (94) | ORF32 (96) | ORF30 (100) | ORF30 (100) | ORF33 (82) | E24 (91) | ORF26 (80) | ORF25 (78) | ORF33 (83) | ||
| − | 31 | 20795 | 20676 | 39 | 4.1 | 8.4 | ATAAATATAGGagaacaaaATG | ORF32 (92) | ORF33 (89) | ORF31 (100) | ORF31 (100) | ORF34 (62) | E23 (89) | ORF27 (79) | ORF26 (79) | ORF34 (79) | ||
| − | 32 | 21095 | 20853 | 80 | 9.7 | 6.7 | CGAAAGGAAGTaaatagATG | ORF33 (88) | ORF35 (88) | ORF32 (100) | ORF32 (100) | E21 (85) | ORF30 (86) | ORF29 (83) | ORF29 (88) | ORF36 (85) | ||
| − | 33 | 21206 | 21096 | 36 | 3.8 | 9.4 | ATAAAGGAGCGatacaATG | ORF36 (100) | ORF33 (100) | ORF33 (100) | E19 (91) | sk1p32 (94) | ORF30 (86) | |||||
| − | 34 | 21555 | 21241 | 104 | 11.9 | 4.6 | ACTATGGTGGTatcgtctaATG | ORF34 (92) | ORF37 (90) | ORF34 (100) | ORF34 (100) | ORF35 (86) | E17 (88) | ORF31 (83) | ORF30 (86) | ORF38 (82) | ||
| − | 35 | 21746 | 21555 | 63 | 7.4 | 6.5 | GGAAAGAGGAAaaATG | ORF35 (93) | ORF38 (90) | ORF35 (100) | ORF35 (100) | |||||||
| − | 36 | 22338 | 21826 | 170 | 20.3 | 9.7 | ATATATGAGGGagtattATG | ORF36 (98) | ORF39 (98) | ORF36 (100) | ORF36 (100) | ORF37 (94) | E15 (91) | ORF32 (91) | ORF31 (91) | ORF32 (96) | ORF39 (91) | |
| − | 37 | 22547 | 22335 | 70 | 8.3 | 4.7 | GGAAAGTTGGTttcATG | ORF37 (95) | ORF40 (92) | ORF37 (100) | ORF37 (100) | ORF38 (92) | E14 (92) | ORF33 (92) | ORF32 (92) | ORF33 (91) | ORF40 (94) | |
| − | 38 | 22922 | 22563 | 119 | 13.0 | 5.0 | AGAGGGAAAATaaaaaATG | ORF38 (100) | ORF41 (90) | ORF38 (100) | ORF38 (100) | ORF39 (92) | E13 (94) | ORF34 (92) | ORF33 (92) | ORF34 (94) | ORF41 (88) | |
| − | 39 | 23549 | 22926 | 207 | 23.9 | 6.7 | CAAATGGAGAAaaaaATG | ORF39 (61) | ORF42 (63) | ORF39 (100) | ORF39 (100) | ORF40 (61) | E12 (92) | ORF35 (96) | ORF34 (95) | ORF35 (63) | ORF42 (91) | Sak protein |
| − | 40 | 24046 | 23546 | 166 | 19.2 | 9.6 | AGAAAGAGGAGaaaATG | ORF40 (100) | ORF40 (100) | E11 (94) | HNH endonuclease | |||||||
| − | 41 | 24299 | 24033 | 88 | 10.5 | 7.8 | TATTAGAAAGTtcatttATG | ORF40 (91) | ORF43 (90) | ORF41 (100) | ORF41 (100) | ORF42 (86) | E10 (94) | ORF38 (76) | ORF37 (84) | ORF38 (76) | ORF43 (88) | |
| − | 42 | 24543 | 24256 | 95 | 11.7 | 10.0 | ATAAAGGAGAAataATG | ORF41 (91) | ORF44 (95) | ORF42 (100) | ORF42 (100) | ORF43 (93) | E9 (92) | ORF39 (95) | ORF38 (97) | ORF44 (93) | ||
| − | 43 | 24853 | 24596 | 85 | 10.1 | 9.1 | AGCAAGGAAAGgtaacagaaaATG | ORF43 (94) | ORF45 (88) | ORF43 (100) | ORF43 (100) | ORF45 (87) | E7 (87) | ORF41 (89) | ORF40 (89) | ORF40 (88) | ORF46 (89) | |
| − | 44 | 25742 | 24846 | 298 | 34.2 | 5.7 | CTAAAGGAGAAagaaATG | ORF44 (45) | ORF47 (45) | ORF44 (100) | ORF44 (100) | ORF47 (96) | E5 (45) | ORF43 (43) | ORF42 (44) | ORF42 (95) | ORF47 (46) | DNA polymerase subunit |
| − | 45 | 25947 | 25786 | 53 | 6.2 | 6.5 | AGAAAAGGAGAaaatATG | ORF45 (90) | ORF45 (100) | ORF48 (92) | ORF45 (76) | |||||||
| − | 46 | 26957 | 26769 | 62 | 7.6 | 9.6 | TTATAGGAGGTaactATG | ORF47 (93) | ORF50 (91) | ORF47 (91) | ORF46 (100) | ORF52 (85) | E1 (90) | ORF50 (88) | ORF45 (85) | ORF49 (85) | ORF50 (91) | |
| + | 47 | 26988 | 27215 | 75 | 8.5 | 4.9 | ATAAAGAGTATaacataaaATG | ORF48 (97) | ORF51 (95) | ORF48 (92) | ORF47 (100) | ORF53 (97) | M1 (95) | ORF51 (95) | ORF46 (95) | ORF51 (90) | ORF51 (95) | |
| + | 48 | 27220 | 27351 | 43 | 5.2 | 4.9 | TTAAGGGAGAAtaaagaaATG | ORF49 (93) | ORF52 (90) | ORF49 (93) | ORF48 (100) | ORF54 (93) | M2 (90) | ORF52 (93) | ORF47 (93) | ORF52 (93) | ORF52 (95) | |
| + | 49 | 27348 | 27827 | 159 | 17.9 | 6.8 | TGAATTGAGTTctgatttATG | ORF50 (96) | ORF53 (95) | ORF50 (96) | ORF49 (100) | ORF55 (96) | M3 (92) | ORF53 (96) | ORF48 (94) | ORF53 (94) | ORF53 (91) | Holliday junction endonuclease |
| + | 50 | 27828 | 27995 | 55 | 6.2 | 6.5 | AGAAAGTCAGGataagtaaATG | ORF51 (96) | ORF54 (91) | ORF51 (96) | ORF50 (100) | ORF56 (98) | M4 (98) | ORF54 (100) | ORF49 (100) | ORF54 (92) | ORF54 (100) | |
| + | 51 | 28416 | 28556 | 46 | 5.3 | 11.2 | AGATAAGGGAGaagcaaATG | ORF52 (97) | ORF55 (86) | ORF52 (86) | ORF51 (100) | ORF58 (76) | ||||||
a
The percentages have been calculated for the smallest proteins. ORFs in bold represent those of the CB19 genome that comprise part of the core genome of the 936 group. aa, amino acids; MM, molecular mass; SD, Shine-Dalgarno.
b
Lowercase bases are not part of the SD sequence.