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. 2009 Jun 19;75(16):5237–5243. doi: 10.1128/AEM.00532-09

FIG. 2.

FIG. 2.

SDS-PAGE map of GST-Bel protein expression. M, protein marker SM0661 (Fermentas); lane 1, E.coli BL21(DE3) containing pGEX-6p-1 vector without isopropyl-β-d-thiogalactopyranoside (IPTG) induction, cell pellet; lane 2, BL21(DE3) containing pGEX-6p-1 vector with 0.8 mM IPTG induction, cell pellet; lane 3, E. coli BL21(DE3) containing pEMB0717 vector without IPTG induction, cell pellet; lane 4, E. coli BL21(DE3) containing pEMB0717 vector with 0.8 mM IPTG induction, cell pellet; lane 5, purified GST-Bel fusion protein eluted from the glutathione Sepharose bulk; lane 6, purified GST tag peptide eluted from the glutathione Sepharose bulk. The acrylamide percentage of the SDS-PAGE gels is 10%.