TABLE 2.
Primer | Sequencea | Product size |
---|---|---|
EUP1 | 5′-GCAGGATCCACCGCCAGCACCAGATAT-3′ | 840 bp |
EUP2 | 5′-GCATCTAGAGTAACTATAATAATTACACT-3′ | |
EDP1 | 5′-GCAGAATTCCCTTCCATTTCCTTCGTAT-3′ | 1.1 kb |
EDP2 | 5′-GCAGGATCCATATCTGGTGCTGGCGGT-3′ | |
belP1 | 5′-GCCGGATCCATGTATACAATGTTTTTCCTC-3′ | 2.2 kb |
belP2 | 5′-GGCGAATTCTTATTCATTATATAAGCTATC-3′ |
The restriction sites included in the oligonucleotide sequence, used during the cloning experiments, are underlined.