TABLE 3.

Percentages of each S. Typhimurium cell type distinguished by a combination of culturability and viability (cytometric analysis) assays

Cell type	% of cellsa
	10 h				24 h				48 h
	Total	LD	MD	HD	Total	LD	MD	HD	Total	LD	MD	HD
Culturable	100	100	100	8	81	100	55	0.6	59	100	43	0.33
VBNC				38	17		38	5	34		53	5
Membrane-damaged				29	2		8	19	3		4	10
DNA-damaged				24				76	4			85

^a“Total” refers to the total population observed after ultracentrifugation and rehomogenization of cells in the centrifuge tube. The VBNC-cell/culturable-cell ratios were as follows: for the HD subpopulation after 10 h of culture, 4.8; for the total population after 24 h of culture, 0.2; for the MD subpopulation after 24 h of culture, 0.69; for the HD subpopulation after 24 h of culture, 8.3; for the total population after 48 h of culture, 0.58; for the MD subpopulation after 48 h of culture, 1.2; and for the HD subpopulation after 48 h of culture, 15.