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. 2009 Jun 26;75(16):5290–5299. doi: 10.1128/AEM.00621-09

FIG. 4.

FIG. 4.

Production of 3HB monomers from in vitro degradation of nPHB granules or dPHB by PHB depolymerases. The reaction mixture (200 μl) contained 0.7 mg of nPHB granules or dPHB. (A) Amounts of 3HB monomers produced from the degradation of nPHB granules divided by the total 3HB equivalent in PHB. Lanes: 1 to 4, amounts produced from the degradation of nPHB granules in the absence of trypsin by 3 μg of B. megaterium PhaZ1 for 5, 10, 30, and 120 min, respectively, and 5, amounts produced from the degradation of trypsin-pretreated nPHB granules by 3 μg of B. thuringiensis PhaZ for 120 min. (B) Amounts of 3HB monomers generated from the degradation of dPHB for 180 min divided by the total 3HB equivalent in PHB. Lanes: 1 and 2, amounts produced from the degradation by 3 and 15 μg of B. megaterium PhaZ1, respectively, and 3 and 4, amounts produced from the degradation by 3 and 15 μg of B. thuringiensis PhaZ, respectively. Each value is the mean of three determinations. Each error bar indicates the standard error of the mean.