FIG. 7.

Relative GUS activity and GUS mRNA accumulation in pβC1 and pINT121 transgenic tobacco plants. The mean GUS activity from the 35S promoter of pINT121 was considered 100% and used to standardize the activity from the promoter in pβC1. Nucleic acid was extracted from the leaves of transgenic plants and analyzed by Northern blotting. Equal amounts of total RNA (15 μg) from each transgenic line were loaded into each lane for the assay, and the blot was hybridized with a probe specific for the GUS gene. Lane 1, untransformed tobacco plant; lanes 2 and 3, plants transformed with pβC1; lanes 4 and 5, plants transformed with pINT121.