TABLE 5.

Mean cycle threshold values of Salmonella serotype Enteritidis (phage types 4, 8, and 13) and Salmonella serotype Typhimurium (phage type DT104) detection from artificially contaminated shell eggs by cell culture and RT-PCR at 5 h postinfection by using orgC and sefA primer and probe sets

Salmonella serotype amt (CFU/ml)	Cycle threshold value for indicated primer/probe seta
	orgC				sefA
	SE4	SE8	SE13	DT104	SE4	SE8	SE13	DT104
107	18.68 ± 0.17	18.13 ± 0.19	17.15 ± 0.58	16.41 ± 0.28	18.61 ± 0.57	18.14 ± 0.26	18.74 ± 0.75	ND
106	19.55 ± 0.73	19.56 ± 0.75	18.16 ± 0.78	17.36 ± 0.38	20.75 ± 0.96	20.83 ± 0.44	20.20 ± 0.35	ND
105	22.52 ± 0.41	22.14 ± 0.81	22.88 ± 1.39	20.70 ± 0.17	24.10 ± 0.46	23.51 ± 0.53	24.78 ± 0.51	ND
104	23.19 ± 0.16	26.69 ± 0.85	26.38 ± 0.51	24.40 ± 0.10	24.75 ± 0.19	28.07 ± 0.37	27.92 ± 1.17	ND
103	26.74 ± 1.11	29.18 ± 0.41	29.65 ± 0.72	27.72 ± 0.07	28.33 ± 1.44	30.49 ± 0.16	30.72 ± 1.44	ND
102	31.15 ± 1.01	30.93 ± 1.15	32.86 ± 2.05	31.80 ± 0.33	32.31 ± 0.97	32.79 ± 0.78	34.48 ± 4.01	ND
101	32.50 ± 2.06	34.91 ± 1.77	36.26 ± 2.55	40.46 ± 0.94	34.06 ± 0.86	34.68 ± 0.72	36.26 ± 3.52	ND
0	ND	ND	ND	ND	ND	ND	ND	ND

^aCycle threshold values are expressed as the means of two replicate experiments performed in duplicate ± standard deviations. ND, not detected.