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. 2009 Jun 26;75(16):5321–5327. doi: 10.1128/AEM.02422-08

TABLE 5.

Mean cycle threshold values of Salmonella serotype Enteritidis (phage types 4, 8, and 13) and Salmonella serotype Typhimurium (phage type DT104) detection from artificially contaminated shell eggs by cell culture and RT-PCR at 5 h postinfection by using orgC and sefA primer and probe sets

Salmonella serotype amt (CFU/ml) Cycle threshold value for indicated primer/probe seta
orgC
sefA
SE4 SE8 SE13 DT104 SE4 SE8 SE13 DT104
107 18.68 ± 0.17 18.13 ± 0.19 17.15 ± 0.58 16.41 ± 0.28 18.61 ± 0.57 18.14 ± 0.26 18.74 ± 0.75 ND
106 19.55 ± 0.73 19.56 ± 0.75 18.16 ± 0.78 17.36 ± 0.38 20.75 ± 0.96 20.83 ± 0.44 20.20 ± 0.35 ND
105 22.52 ± 0.41 22.14 ± 0.81 22.88 ± 1.39 20.70 ± 0.17 24.10 ± 0.46 23.51 ± 0.53 24.78 ± 0.51 ND
104 23.19 ± 0.16 26.69 ± 0.85 26.38 ± 0.51 24.40 ± 0.10 24.75 ± 0.19 28.07 ± 0.37 27.92 ± 1.17 ND
103 26.74 ± 1.11 29.18 ± 0.41 29.65 ± 0.72 27.72 ± 0.07 28.33 ± 1.44 30.49 ± 0.16 30.72 ± 1.44 ND
102 31.15 ± 1.01 30.93 ± 1.15 32.86 ± 2.05 31.80 ± 0.33 32.31 ± 0.97 32.79 ± 0.78 34.48 ± 4.01 ND
101 32.50 ± 2.06 34.91 ± 1.77 36.26 ± 2.55 40.46 ± 0.94 34.06 ± 0.86 34.68 ± 0.72 36.26 ± 3.52 ND
0 ND ND ND ND ND ND ND ND
a

Cycle threshold values are expressed as the means of two replicate experiments performed in duplicate ± standard deviations. ND, not detected.