FIG. 2.

Growth and substrate consumption in vanA1B and pcaG1H1 mutants of B. japonicum USDA110. (A and B) Physical maps of vanA1B (A) and pcaG1H1 (B) deletion mutants. Cell growth was monitored by measuring turbidity (optical density at 660 nm [OD₆₆₀]). (C and F) The B. japonicum cells were grown in minimal medium supplemented with vanillate (C and E) or with protocatechuate (D and F). The concentrations of vanillate (E) and protocatechuate (F) in culture were determined by HPLC. The error bars indicate standard deviations of triplicate determinations. WT, wild type. Dashed lines indicate cross-combinations of mutated genes and substrate (pcaG1H1 mutant in vanillate-amended medium [E], vanA1B mutant in protocatechuate-amended medium [F]).