Skip to main content

View full-text article in PMC

. 2009 Jun 5;75(15):4993–5000. doi: 10.1128/AEM.02917-08

TABLE 3.

Reaction components of a 25-μl Mastermix assay used for quantification of the functional target genes involved in nitrogen turnover

Target metabolic group	Target gene	Amt (μl) of assay component
		2× Power SYBR green	3% BSA	Primer (10 pmol μl⁻¹)		Dimethyl sulfoxide	DNA	PCR water
		2× Power SYBR green	3% BSA	Forward	Reverse	Dimethyl sulfoxide	DNA	PCR water
Nitrogen-fixing bacteria	nifH	12.5	0.5	0.30	0.30		2	9.4
AOB	amoA	12.5	0.5	0.75	0.75		2	8.5
AOA	amoA	12.5	0.5	0.50	0.50		2	9.0
Denitrifying bacteria	nirK	12.5	0.5	0.50	0.50	0.625	2	8.375
	nirS	12.5	0.5	0.50	0.50	0.625	2	8.375