TABLE 5.
Statistical evaluation of gene abundance by multifactorial ANOVA
Factor |
Pa
|
||||
---|---|---|---|---|---|
nifH | amoA (AOB) | amoA (AOA) | nirS | nirK | |
Total | |||||
EC | 0.00016* | 0.00187* | 0.00000* | 0.00000* | 0.00000* |
Organic | 0.00584* | 0.00010* | 0.00663* | 0.01226* | 0.00110* |
Urea | 0.86490 | 0.00000* | 0.76170 | 0.03195* | 0.00897* |
EC × organic | 0.01611* | 0.06726 | 0.75901 | 0.00226* | 0.02470* |
EC × urea | 0.97056 | 0.01246* | 0.94151 | 0.77689 | 0.26311 |
Organic × urea | 0.88557 | 0.91020 | 0.40479 | 0.48152 | 0.67149 |
EC × organic × urea | 0.96940 | 0.34970 | 0.70371 | 0.11885 | 0.20102 |
EC30 | |||||
Organic | 0.21544 | 0.01357* | 0.01120* | 0.00461* | 0.00226* |
Urea | 0.90946 | 0.00011* | 0.92578 | 0.08370 | 0.03994* |
Organic × urea | 0.95764 | 0.50181 | 0.41531 | 0.54840 | 0.31779 |
EC60 | |||||
Organic | 0.00028* | 0.01036* | 0.44637 | 0.00033* | 0.12915 |
Urea | 0.91401 | 0.00004* | 0.76804 | 0.44207 | 0.79858 |
Organic × urea | 0.75057 | 0.43477 | 0.69985 | 0.98800 | 0.68548 |
EC90 | |||||
Organic | 0.78713 | 0.06200 | 0.12951 | 0.48282 | 0.72698 |
Urea | 0.62794 | 0.000109* | 0.74827 | 0.19272 | 0.01398* |
Organic × urea | 0.71996 | 0.30539 | 0.19268 | 0.08998 | 0.07509 |
The P values describe the impact of plant development stages and nitrogen fertilizing treatments on functional genes involved in nitrogen turnover. Asterisks indicate significant effects of plant development stages or fertilizing treatments, respectively, on the functional genes, after analysis by Tukey's test with SPSS 13.0.