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kcat/Km (s−1 M−1) values for PTEAr and variants with different OPsa
| Substrate |
kcat/Km (s−1 M−1) for PTEAr:
|
|||
|---|---|---|---|---|
| WT | F132A | W131H | W131H/F132A | |
| MPS | 2.7 × 107 | 1.8 × 106 | 1.3 × 106 | 8.7 × 105 |
| DCV | 8.1 × 105 | NA | NA | NA |
| Z-CVP | 9.6 × 10° | 3.9 × 103 | 2.4 × 103 | 4.6 × 103 |
| E-CVP | 8.4 × 102 | 4.3 × 103 | 3.1 × 103 | 6.0 × 103 |
MPS, methyl parathion (ChemService); DCV, dichlorvos (ChemService); CVP, chlorfenvinphos (Bayer Crop Science, Australia); WT, wild type; NA, not available. Error is within 10%.
