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Flagellation of Δfla, ΔmotAB, and ΔcheY-3 mutants. Bacteria from LB-N soft-agar plates were stained with Nano Orange and examined using epifluorescence at a ×1,000 magnification as described in Materials and Methods. (A) Wild-type CMCP6; (B) ΔflaFBA::cat strain FLA680; (C) ΔflaCDE::aph strain FLA677; (D) ΔflaCDE::aph ΔflaFBA::cat strain FLA810; (E) ΔmotAB::aph strain FLA674; (F) ΔcheY-3::aph strain FLA688.
