TABLE 1.
Bacterial strains used in this work
| Strain | Relevant genotype or descriptiona | Source or reference |
|---|---|---|
| E. coli | ||
| S17-1λpir | RP4 2-Tc::Mu-Km::Tn7 pro thi recA HsdR−M+ λpir | 39 |
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| EC100Dpir+ | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG pir+(DHFR) | Epicentre |
| V. vulnificus | ||
| CMCP6 | Wild type, biotype 1 clinical isolate | 20 |
| M06-24/O | Wild type, biotype 1 clinical isolate | 49 |
| CVD752 | M06-24/O wza::TnphoA | 48 |
| FLA591 | CMCP6 ΔrtxC; constructed using pGTR265 | This work |
| FLA614 | CMCP6 ΔfadR::aph; constructed using pGTR2010 | 6 |
| FLA674 | CMCP6 ΔmotAB::aph; constructed using pGTR1141 | This work |
| FLA677 | CMCP6 ΔflaCDE::aph; constructed using pGTR1136 | This work |
| FLA680 | CMCP6 ΔflaFBA::cat; constructed using pGTR1120 | This work |
| FLA688 | CMCP6 ΔcheY-3::aph; constructed using pGTR1171 | This work |
| FLA711 | ΔflaCDE::aph ΔflaFBA::cat; constructed in FLA680 using pGTR1136 via conjugation and two-step allelic exchange mutagenesis | This work |
| FLA810 | ΔflaCDE::aph ΔflaFBA::cat; constructed using chitin transformation of genomic DNA from FLA677 into FLA680 | This work |
| FLA900 | CMCP6 ΔrtxA1::aph; constructed using pGTR274 | This work |
| FLA1009 | CMCP6 wza::TnphoA; constructed by chitin transformation using genomic DNA of CVD752 | This work |
a
DHFR, dihydrofolate reductase.