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. Author manuscript; available in PMC: 2009 Aug 12.
Published in final edited form as: Cell Cycle. 2006 Jun 1;5(11):1184–1188. doi: 10.4161/cc.5.11.2804

Figure 4. Dpp is active in the absence of rasp.

Figure 4

All panels show third instar wing discs. (A–C) wildtype; (D–F) raspT392/raspT802; (G–I) raspT392/raspT802; vg-GAL4/UAS-dpp. The discs were stained to show expression of the Dpp target genes optomotor-blind (Omb; magenta in A, C, D, F, G, I) and spalt (Sal; green in B, C, E, F, H, I). dpp is not expressed in rasp mutant discs due to defective Hh signaling (6 and see Fig. 3); however, when misexpressed at the dorsal-ventral boundary of the wing disc it is able to activate sal and omb expression. Transfections and X-gal and antibody stainings were carried out as described11.