FIG. 1.

Induction of IFNs and IFN type I-responsive genes by CpG formulated in Emulsigen. PBMCs were injected intramuscularly with CpG ODN 2142 formulated in Emulsigen at 0.5 or 2 mg per animal, or not injected into control animals (ctrl), and data were collected at 24 h postinjection. After total RNA was harvested, IFNs (α, β, and γ), and mRNA levels of IFN-responsive genes (Mx1, MyD88, OAS, PKR, IRF-3, IRF-7, RPL32, and PPLA) were quantified by real-time PCR. The mRNA levels were normalized to 18S mRNA levels and are expressed as fold induction over the samples obtained before injection. The bars represent individual pigs (two for each group).