FIG. 2.

Induction of OAS, IRF7, and Mx1 mRNAs by CpG formulated in the DOE-FMD vaccine or Emulsigen. PBMCs from pigs (n = 2) injected with the DOE-FMD (conventional commercial FMD vaccine), CpG formulated in DOE-FMD, or CpG formulated in Emulsigen (Emul-CpG) were isolated at 24, 48, and 72 h postinjection. Total RNA was harvested and quantified by real-time PCR and normalized to 18S mRNA levels. For CpG injections, 0.5 mg of ODN 2142 was formulated and injected in a total of 2 ml. The fold induction of target genes was calculated based on mRNA levels before injection. The averages from three independent experiments with standard deviation error bars are shown.