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Western blot analysis. rBCG-AN-E-AC lysates and culture supernatants were analyzed for expression of the chimeric protein by immunoblotting with antibodies recognizing Ag85B (a) and ESAT-6 (b). Lanes: 1, cell lysates of rBCG-AN-E-AC; 2, culture supernatants of rBCG-AN-E-AC; 3, culture supernatants of BCG.
