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Analysis of T-cell percentages. Splenocytes were extracted from the immunized mice and incubated with FITC-conjugated anti-CD4 and PE-conjugated anti-CD8. The percentages of CD4+ and CD8+ T cells were determined by flow cytometry. The results are expressed as the mean ± standard error. *, the percentages of CD4+ and CD8+ T cells were significantly greater than those in the group immunized with PBS or BCG (P < 0.05).
