TABLE 5.

Specificity of the anti-HBs in-house assay compared to the AUSAB EIA

AUSAB EIA antibody concn (mIU/ml) before preincubation	Anti-HBs in-house assay antibody concn (mIU/ml) before preincubation	HBsAg preincubation with subtype(s)a:
		ad + ay				adw2
		AUSAB EIA		Anti-HBs in-house assay		AUSAB EIA		Anti-HBs in-house assay
		Antibody concn (mIU/ml)	% Specificity	Antibody concn (mIU/ml)	% Specificity	Antibody concn (mIU/ml)	% Specificity	Antibody concn (mIU/ml)	% Specificity
20	15	<3.3	100	<3.3	100	<3.3	100	<3.3	100
94	61	<3.3	100	<3.3	100	<3.3	100	<3.3	100
119	125	<3.3	100	<3.3	100	<3.3	100	<3.3	100
346	352	<3.3	100	<3.3	100	<3.3	100	<3.3	100
382	428	<3.3	100	<3.3	100	<3.3	100	<3.3	100
1,556	1,111	<3.3	100	<3.3	100	<3.3	100	<3.3	100
1,957	2,410	<3.3	100	<3.3	100	<3.3	100	<3.3	100
2,237	2,575	<3.3	100	<3.3	100	<3.3	100	<3.3	100
49,680	42,660	<3.3	100	<3.3	100	86	99.8	49	99.9
54,490	61,065	<3.3	100	<3.3	100	651	98.8	103	99.8
<3.3	<3.3	<3.3	NA	<3.3	NA	<3.3		<3.3
<3.3	<3.3	<3.3	NA	<3.3	NA	<3.3		<3.3
<3.3	<3.3	<3.3	NA	<3.3	NA	<3.3		<3.3

^aPreincubation was performed with either an excess of a mix of native ad and ay HBsAg subtypes or adw2 HBsAg subtype for 2 h at 37°C. Specificity represents the difference in concentrations observed before and after inhibition expressed as a percentage of the corresponding concentration measured without inhibition.