TABLE 2.

Plasmids used in this study

Name	Yeast marker(s)	Promoter	Cloned gene	Source or reference
pUN40	TRP1, CEN			11
pUN30-SSO1	TRP1, CEN	SSO1	SSO1plus 500 bp 3′	This study
pUN50-SSO1	URA3, CEN	SSO1	SSO1plus 500 bp 3′	This study
pUN50-SSO1R45A R63D	URA3, CEN	SSO1	SSO1plus 500 bp 3′	This study
pUN40-SSO2	TRP1, CEN	SSO2	SSO2plus 300 bp 3′	This study
pUN40-SSO2A49R D67R	TRP1, CEN	SSO2	SSO2plus 300 bp 3′	This study
pRS424	TRP1, 2μ			51
pRS424-SSO1	TRP1, 2μ	SSO1	SSO1plus 500 bp 3′	This study
YEp352	URA3, 2μ			17
YEp352-SSO1	URA3, 2μ	SSO1	SSO1plus 500 bp 3′	This study
YEp352-SSO2	URA3, 2μ	SSO2	SSO2plus 300 bp 3′	This study
YEp352-SSO2A49R D67R	URA3, 2μ	SSO2	SSO2plus 300 bp 3′	This study
YEp-MSS4-URA	URA3, 2μ	MSS4	MSS4	48
YEp-MSS4-TRP	TRP1, 2μ	MSS4	MSS4	48
YEp-SPO14	TRP1, 2μ	SPO14	SPO14	47
pRS424-TEF2pr-GFP-SSO1	TRP1, 2μ	TEF2	GFP-SSO1	40
pRS424-SPO20pr-GFP-SSO1	TRP1, 2μ	SPO20	GFP-SSO1plus 500 bp 3′	40
pRS424-TEF2pr-GFP-SSO2	TRP1, 2μ	TEF2	GFP-SSO2	This study
pRS424-SPO20pr-GFP-SSO2	TRP1, 2μ	SPO20	GFP-SSO2plus 500 bp 3′	This study
pRS424-G20	TRP1, 2μ	DTR1	GFP-SPO2051-91	40
pRS306-MPC54-RFP	URA3, integration	MPC54	RFP-MPC54	40
pKR466	URA3, integration			45
pGEX-5X-1			GST	52
pGEX-SSO1-TMD			GST-SSO11-265	J. Jäntti
pGEX-SSO2-TMD			GST-SSO21-269	J. Jäntti
pGEX-SSO1-start-HLH			GST-SSO11-148	This study
pGEX-SSO1-Habc			GST-SSO132-148	This study
pGEX-SSO1-HabcR45A R63D			GST-SSO132-148	This study
pGEX-SSO1-HLH			GST-SSO1149-192	This study
pGEX-SSO1-HLH-SNARE			GST-SSO1149-264	This study
pGEX-SSO1-SNARE			GST-SSO1194-264	This study
pGEX-SSO2-Habc			GST-SSO235-152	This study
pGEX-SSO2-HabcA49R D67R			GST-SSO235-152	This study
pGEX-SSO2-SNARE			GST-SSO2196-268	This study