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In vivo hydrolysis of internalized BODIPY-PC
| Strain | Relevant genotype | % BODIPY-PAa |
|---|---|---|
| AN120 | SPO14SSO1 | 2.5 ± 0.7 |
| Y4451 | spo14Δ | 0.1 ± 0.1 |
| Y4454 | sso1Δ | 3.2 ± 1.1 |
| Y4459 | spo14Δ sso1Δ | 0.1 ± 0.0 |
The percent conversion of internalized BODIPY-PC to BODIPY-PA was determined in cells induced to sporulate at 30°C, as described in Materials and Methods. Values are means ± SEM for three independent experiments.
