TABLE 6.
Sporulation in wild-type and sso1Δ cells
| Strain | Relevant genotypea | % Spore formation (mean ± SD)b |
|---|---|---|
| Y8230 | SSO1 (SSO1 CEN) | 81.0 ± 9.7 |
| Y8232 | SSO1(SSO2 CEN) | 93.4 ± 1.8 |
| Y8234 | SSO1(CEN) | 84.2 ± 2.5 |
| Y8262 | sso1Δ (SSO1 CEN) | 61.8 ± 3.6 |
| Y8238 | sso1Δ (SSO2 CEN) | <0.1 |
| Y8240 | sso1Δ (CEN) | <0.1 |
| Y8121 | SSO1(SSO1 2μ) | 82.6 ± 9.2 |
| Y8124 | SSO1(SSO2 2μ) | 67.6 ± 4.0 |
| Y8127 | SSO1(2μ) | 90.0 ± 0.7 |
| Y8265 | sso1Δ (SSO1 2μ) | 72.4 ± 4.6 |
| Y8116 | sso1Δ (SSO2 2μ) | 8.0 ± 5.2 |
| Y8119 | sso1Δ (2μ) | <0.1 |
| Y8208 | SSO1(MSS4 2μ) | 79.0 ± 6.1 |
| Y8211 | SSO1(2μ) | 82.8 ± 3.6 |
| Y8214 | sso1Δ (MSS4 2μ) | 0.3 ± 0.1 |
| Y8217 | sso1Δ (2μ) | <0.1 |
| Y8220 | SSO1(MSS4 2μ SPO14 2μ) | 74.0 ± 7.5 |
| Y8222 | SSO1 (SPO14 2μ) | 67.8 ± 4.5 |
| Y8224 | sso1Δ (MSS4 2μ SPO14 2μ) | 0.2 ± 0.2 |
| Y8226 | sso1Δ (SPO14 2μ) | <0.1 |
| Y8159 | sso1Δ (MSS4 2μ SSO2CEN) | 0.2 ± 0.2 |
| Y8298 | sso1Δ (MSS4 2μ SSO2 2μ) | 21.3 ± 6.1 |
| Y8319 | sso1Δ (sso1R45A R63DCEN) | 73.4 ± 6.9* |
| Y8294 | sso1Δ (sso2A49R D67RCEN) | <0.1* |
| Y8347 | sso1Δ (sso2A49R D67R 2μ) | 21.1 ± 8.3* |
a
Genes in parentheses are carried by the plasmids.
b
A total of 300 and 1,000 cells were counted from three independent experiments for SSO1 and sso1Δ strains, respectively. Asterisks indicate strains in which 500 cells were counted per experiment. Sporulation was induced on solid medium. All cells with 1 to 4 spores visible by differential interference contrast optics were included.