FIG. 1.

Eng2 accumulates during sporulation. (A) Western blot analysis of Eng2-GFP during vegetative growth. Samples were collected from exponentially growing cultures of wild-type (WT; OL23), eng2Δ mutant (OL24), and eng2-GFP (OL896) strains to prepare protein extracts. Anti-GFP antibody was used. (B) Western blot analysis of Eng2-GFP and Agn2-GFP during sporulation. The diploid strains OL961 and OL962 were induced to sporulate. Samples were collected at the indicated times after the induction of sporulation to prepare protein extracts. Anti-GFP antibody was used. Actin was used as a loading control. Meiotic progression was followed by DAPI staining of nuclei, and sporulation was checked by microscopic observation of asci. The percentages of mononucleate, binucleate, and tetranucleate cells and spores at each time point for the strain carrying Eng2-GFP are shown in the graph.