FIG. 2.

β-1,3-Glucanase activity during sporulation. (A) The diploid strains OL946 (WT), OL948 (eng1Δ/eng1Δ), and OL950 (eng2Δ/eng2Δ) were grown on EMM-AC and then transferred to EMM-SG to induce sporulation. Samples were collected at the indicated times after the induction of sporulation to prepare protein extracts and to assay β-1,3-glucanase activity, using laminarin as substrate. Activity is represented as units/mg protein. Values are means of results from three independent measurements, and standard deviations are shown. MI, meiosis I; MII, meiosis II. (B) Meiotic progression of the wild-type (WT) and eng2Δ/eng2Δ strains. Aliquots of the culture were stained with DAPI, and the percentages of mononucleate, binucleate, and tetranucleate cells and spores at each time-point are shown. Images show mature spores after 24 h of incubation in sporulation medium.