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Eng2 localizes to the cytoplasm of the ascus. (A) Haploid Eng2-GFP (OL952) or Agn2-GFP (OL954) cells were allowed to mate on sporulation plates with cells from the opposite mating type carrying the same tagged proteins (strain OL953 or OL955, respectively), and the resulting zygotic asci were examined using fluorescence microscopy. Bar, 10 μm. (B) Details of free spores. (C) Germination of spores from the wild-type (WT) and eng2Δ/eng2Δ crosses. DIC, differential interface contrast.
