FIG. 3.

Western blot analysis of BTS-GFP transfectant lines. Predicted molecular masses of preproteins are presented. Based on these predictions, an asterisk indicates the unprocessed preprotein (after removal of signal peptide), a diamond indicates the processed mature protein, and an arrow indicates unspecific GFP degradation products. No immunoreactive bands are detected in protein samples prepared from the parental 3D7 line. LE, long exposure to reveal higher-molecular-mass bands in 3D7^sCdc48-BTS samples. The values for the predicted molecular weight (MW) after removal of the signal peptide (−SP) are given in kDa.