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HPLC analysis of sterols extracted from WT T. thermophila and the KO270 mutant grown with lathosterol at zero (T0) and 24 (T24) h. For quantification, stigmasterol (cholest-5,22-dien-24β-ethyl-3β-ol) was added in all cases as an internal standard. Absorbance was recorded at 210 nm for all sterols and 285 nm for sterols displaying conjugated double bonds (5,7-diene derivatives).
