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GC-MS analysis of trimethylsilyl ether derivatives of total sterols isolated from WT T. thermophila and the KO270 mutant grown with lathosterol. (A) Sterols were recovered after 24 h of culturing. (B) Mass spectra of cholest-5,7,22-trien-3β-ol and (5α)-cholest-7,22-dien-3β-ol trimethylsilyl derivatives. The compounds were identified with the National Institute of Standards and Technology library.
