TABLE 3.
Sterol biotransformations expected in the T. thermophila WT and KO270 knockout mutanta
| Strain | Products in cultures supplemented with:
|
||
|---|---|---|---|
| Cholesterol | Cholestanol | Lathosterol | |
| WT | Cholest-5, 22-dien-3β-ol; cholest-5, 7-dien-3β-ol; cholest-5, 7, 22-trien-3β-ol (↑) | 5α-Cholest-22-en-3β-ol; lathosterol; cholesterol; cholest-5, 7-dien-3β-ol; cholest-5, 22-dien-3β-ol; 5α-cholest-7, 22-dien-3β-ol; cholest-5, 7, 22-trien-3β-ol (↑) | Cholest-5, 7-dien-3β-ol; cholest-5, 22- dien-3β-ol; cholest-5, 7, 22-trien-3β-ol (↑) |
| KO270 | Cholest-5, 22-dien-3β-ol; cholest-5, 7-dien-3β-ol; cholest-5, 7, 22-trien-3β-ol (↑) | 5α-Cholest-22-en-3β-ol; lathosterol; cholesterol (↓); cholest-5, 7-dien-3β-ol (↓); cholest-5, 22-dien-3β-ol (↓); 5α-cholest-7, 22-dien-3β-ol (↑); cholest-5, 7, 22-trien-3β-ol (↓) | Cholest-5, 7-dien-3β-ol (↓); 5α-cholest-7, 22-dien-3β-ol (↑); cholest-5, 7, 22-trien-3β-ol (↓) |
a
Arrows indicate whether an increase (↑) or decrease (↓) of the products is expected.