TABLE 4.
Recovery of sterols from WT T. thermophila and mutant KO270 cultured with cholesterol or lathosterola
| Sterol | Amount (in μg/ml) (%) of sterol at:
|
|||
|---|---|---|---|---|
| 0 h
|
24 h
|
|||
| WT | KO270 | WT | KO270 | |
| Cholesterol | 21.74 (100) | 18.82 (100) | 7.02 (32) | 8.32 (44) |
| Cholest-5, 7-dien-3β-ol | <0.05 (<1) | <0.05 (<1) | 0.84 (4) | 0.42 (2) |
| Cholest-5, 7, 22-trien-3β-ol | <0.05 (<1) | <0.05 (<1) | 13.46 (61) | 10.26 (55) |
| Lathosterol | 19.42 (100) | 22.54 (100) | 9.02 (46) | 12.03 (54) |
| Cholest-5, 7-dien-3β-ol | <0.05 (<1) | <0.05 (<1) | 2.8 (14) | <0.05 (<1) |
| Cholest-5, 7, 22-trien-3β-ol | <0.05 (<1) | <0.05 (<1) | 7.1 (36) | 1.46 (6) |
a
Cells were grown in 250-ml Erlenmeyer flasks containing 100 ml of SPP medium supplemented with cholesterol or lathosterol at 20 μg/ml (final concentration). Results shown are mean values from three independent experiments.