TABLE 3.
Plasmids used in this study
| Vector | Backbone | Description | Source or reference |
|---|---|---|---|
| pBluescript KS(+) | Cloning vector | Stratagene, La Jolla, CA | |
| pDS178 | pRC2312 | pRC2312-derived plasmid containing URA3 and LEU2 | 8 |
| pDS1097 | pDS178 | Insertion of TAC1-1 between XhoI and BamHI sites | 6 |
| pDS1099 | PDS178 | Insertion of TAC1-5 between XhoI and BamHI sites | 6 |
| pDS961 | pBS-KS(+) | Insertion of ADE2 | This study |
| pAC3 | pDS961 | Insertion of P(CDR2) (−720-1)-HIS3 fusion between XhoI and PstI sites | This study |
| pAC4 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-CDR2-mini and HIS3-KPN | This study |
| pAC27 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegpolyT and HIS3-KPN | This study |
| pAC28 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegw/oTc and HIS3-KPN | This study |
| pAC29 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg-CGG1 and HIS3-KPN | This study |
| pAC30 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg-AAA and HIS3-KPN | This study |
| pAC31 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg-CGG2 and HIS3-KPN | This study |
| pAC32 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg-ATA and HIS3-KPN | This study |
| pAC33 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg-part1and HIS3-KPN | This study |
| pAC34 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg-part2 and HIS3-KPN | This study |
| pAC35 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg delete and HIS3-KPN | This study |
| pAC37 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-degV1 and HIS3-KPN | This study |
| pAC38 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-degV2 and HIS3-KPN | This study |
| pAC39 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-degTT and HIS3-KPN | This study |
| pAC40 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdegPoly T1 and HIS3-KPN | This study |
| pAC41 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdegPoly T2 and HIS3-KPN | This study |
| pAC42 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdegPoly T3 and HIS3-KPN | This study |
| pAC43 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdegPoly T4 and HIS3-KPN | This study |
| pAC44 | pDS961 | Insertion of P(CDR2)w/oDRE-HIS3 amplified from pAC3 by fusion of the PCR products from CDR2 nsi- with CDR2 DEL3 and HIS-KPN with CDR2 DEL5 | This study |
| pAC45A,G,Ta | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegC1 and HIS3-KPN | This study |
| pAC46C,T,Aa | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegG1 and HIS3-KPN | This study |
| pAC47C,T,Aa | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegG2 and HIS3-KPN | This study |
| pAC48G,C,Ta | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegA1 and HIS3-KPN | This study |
| pAC49G,C,Ta | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegA2 and HIS3-KPN | This study |
| pAC50G,C,Ta | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegA3 and HIS3-KPN | This study |
| pAC51G,C,Aa | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegTC and HIS3-KPN | This study |
| pAC52A,G,Ta | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegC2 and HIS3-KPN | This study |
| pAC53C,T,Aa | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegG3 and HIS3-KPN | This study |
| pAC54C,T,Aa | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegG4 and HIS3-KPN | This study |
| pAC55G,C,Ta | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegA4 and HIS3-KPN | This study |
| pAC56G,C,Aa | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegT1 and HIS3-KPN | This study |
| pAC57G,C,Ta | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREIIdegA5 and HIS3-KPN | This study |
| pAC126 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-PDR16-zcb-1 and HIS3-KPN | This study |
| pAC127 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-TAC1-501 and HIS3-KPN | This study |
| pAC128 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-TAC1-457 and HIS3-KPN | This study |
| pAC129 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-CDR1 and HIS3-KPN | This study |
| pAC130 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-RTA3 and HIS3-KPN | This study |
| pAC131 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DRE-IFU5 and HIS3-KPN | This study |
| pAC133 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg1T and HIS3-KPN | This study |
| pAC134 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg2T and HIS3-KPN | This study |
| pAC135 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg3T and HIS3-KPN | This study |
| pAC136 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg4T and HIS3-KPN | This study |
| pAC137 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg5T and HIS3-KPN | This study |
| pAC138 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg6T and HIS3-KPN | This study |
| pAC139 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg7T and HIS3-KPN | This study |
| pAC140 | pDS961 | Insertion of P(CDR2)-HIS3 amplified from pAC3 with DREdeg8T and HIS3-KPN | This study |
| CIpACT-C-ZZ | Clp10 | 3 | |
| pAC9 | CIpACT-C-ZZ | Insertion of TAC1 ORF amplified with SPHI-ZINC2C and SALI-ZINC2C | This study |
| pAC218 | pDS961 | Insertion of P(IFU5)(−999-1)-HIS3 between XhoI and KpnI sites | This study |
a
These plasmids have three versions, each containing one of the bases indicated.